讲座题目：Plasmonic nanobiosensors: From therapeutic drug and environmental monitoring to optophysiology of living cells

报告人：Prof. Jean-Francois Masson, Université de Montréal (加拿大蒙特利尔大学化学系)

报告时间：2018年4月30日（星期一）上午9:00

报告地点：国工楼B3111

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报告人简介: Jean-Francois Masson教授于2006年在美国亚利桑那州立大学（Arizona State University）获应用化学专业博士学位，现为加拿大蒙特利尔大学化学系终身教授。曾获加拿大弗瑞德-比米什奖、德国亚历山大·冯·洪堡基金及美国托马斯-赫希菲尔德奖等多项国际奖项；作为主席或主要负责人承办国际会议8次，在国际会议邀请学术报告100余次；已在国际重要纳米、化学及传感器学术期刊发表论文200余篇；承担和主持加拿大、中国、美国及德国等重要研究课题多项，资助经费总计达600多万加元。

报告内容简介：This presentation will provide an overview of our research activities in plasmonic nanobiosensing. Our research lies in the areas of plasmonic materials, low-fouling surface chemistry and instrumental design for biosensing. This presentation will focus on applying these concepts for several classes of sensors for monitoring biomolecules, therapeutic drugs, pheromones and for environmental contaminants. We have developed a SPR and LSPR sensing platform based on a small and portable instrument that can be field-deployed. In the first example, this SPR chip was integrated with a RDX-selective molecularly imprinted polymer to detect RDX at ppb levels directly in natural waters. The system was deployed to a Canadian army base for monitoring the level of RDX in proximity of training grounds. This system was tested on several trips in different environmental conditions and results were in good agreement with HPLC performed in a laboratory. Clinical sensing in crude biofluids is a common challenge to different biosensing platforms. To prevent nonspecific adsorption of serum, a series of peptide monolayers were synthesized and tested in crude serum. Based on this, competition assays were validated for therapeutic drug quantitation, such as methotrexate with the SPR sensors. The methotrexate assay was tested at a local hospital and was cross-validated with the current state-of-the-art FPIA analyzer commercially available. Lastly, we are currently exploring the concept of optophysiology using plasmonic nanopipettes for monitoring living cell secretion events. Due to the lack of analytical techniques for detecting metabolites near living cells, developing tools to monitor cell secretion events remains a challenge to overcome in chemical analysis. Plasmonic nanopipettes were developed based on the decoration of patch clamp nanocapillaries with Au nanoparticles. The plasmonic nanopipette is thus competent for dynamic SERS measurements in the liquid environment near cells. This nanobiosensor was tested with the detection of small metabolites near living MDCKII cells and of neurotransmitters released by neurons.This presentation will provide an overview of our research activities in plasmonic nanobiosensing. Our research lies in the areas of plasmonic materials, low-fouling surface chemistry and instrumental design for biosensing. This presentation will focus on applying these concepts for several classes of sensors for monitoring biomolecules, therapeutic drugs, pheromones and for environmental contaminants. We have developed a SPR and LSPR sensing platform based on a small and portable instrument that can be field-deployed. In the first example, this SPR chip was integrated with a RDX-selective molecularly imprinted polymer to detect RDX at ppb levels directly in natural waters. The system was deployed to a Canadian army base for monitoring the level of RDX in proximity of training grounds. This system was tested on several trips in different environmental conditions and results were in good agreement with HPLC performed in a laboratory. Clinical sensing in crude biofluids is a common challenge to different biosensing platforms. To prevent nonspecific adsorption of serum, a series of peptide monolayers were synthesized and tested in crude serum. Based on this, competition assays were validated for therapeutic drug quantitation, such as methotrexate with the SPR sensors. The methotrexate assay was tested at a local hospital and was cross-validated with the current state-of-the-art FPIA analyzer commercially available. Lastly, we are currently exploring the concept of optophysiology using plasmonic nanopipettes for monitoring living cell secretion events. Due to the lack of analytical techniques for detecting metabolites near living cells, developing tools to monitor cell secretion events remains a challenge to overcome in chemical analysis. Plasmonic nanopipettes were developed based on the decoration of patch clamp nanocapillaries with Au nanoparticles. The plasmonic nanopipette is thus competent for dynamic SERS measurements in the liquid environment near cells. This nanobiosensor was tested with the detection of small metabolites near living MDCKII cells and of neurotransmitters released by neurons.